How to make a large 30 KB plasmid from scratch?

2018-03-13 20:19:44

Whats the process to make a comparably large DNA sequence from scratch.

That is to say, how does one go from a text file with 35 thousand bases to a plasmid ready to transfection a cell?

Specifically, I'm curious how this is done in practice for for larger sequences, on a more industrial scale.